Overview of the ENCODE DNA Trackhub

The ENCODE DNA Trackhub contains signals and peaks produced by the ENCODE Consortium for genome-wide assays related to DNA and chromatin-associated protein factors (mostly transcription factors or TFs), produced till Feb 1, 2019. Included assays profile Chromatin accessibility (ATAC-seq and DNase-seq), occupancy of histone modifications and variants (ChIP-seq), binding by transcription factors (ChIP-seq), DNA methylation (whole-genome bisulfite sequencing or WGBS and reduced-representation bisulfite sequencing or RRBS), and DNA replication patterns (Repli-seq). All data included in this trackhub were produced by ENCODE uniform processing pipelines for the respective assays and are publicly available at the ENCODE Portal. In each dataset, peaks are the genomic regions with significantly high signals. The peaks in this trackhub constitute a component of the ground-level of the ENCODE Encyclopedia. The hub contains data mapped to both the human genome (versions GRCh38 and hg19) and the mouse genome (version mm10) and is updated periodically as more data are produced by ENCODE. To facilitate navigation, we have organized the hub in three ways: (1) all data are grouped by assay and further sub-grouped by biosample for each assay, in the “ATAC-seq”, “DNase-seq”, “Histone ChIP-seq (by biosample)”, and “TF ChIP-seq (by biosample)” composite tracks; (2) ChIP-seq experiments are grouped by the histone or protein factor that the antibody in the ChIP assay targets, in the “TF ChIP-seq (by target)” and “Histone ChIP-seq (by target)” composite tracks; (3) data from all assays are grouped by tissue ontology, defined with metadata obtained from the ENCODE Portal, in the "Experiments by Ontology" composite track.


A related trackhub named “ENCODE RNA Trackhub” contains signals and peaks produced by the ENCODE Consortium for genome-wide assays related to RNA and associated protein factors (mostly RNA-binding proteins or RBPs) produced till Feb 1, 2019.

The “ENCODE DNA Trackhub” and the “ENCODE RNA Trackhub” contain the raw data and output from the ENCODE uniform processing pipelines for individual experiments. Annotations from integrated analyses of multiple experiments, such as a Registry of candidate cis-regulatory elements (cCREs), transcription factor motif sites, and other integrated-level components of the ENCODE Encyclopedia are available in a separate, associated trackhub named “ENCODE Integrative Annotation Trackhub”.

Available assays:

The following assays have data available. See ENCODE data processing pipeline description for more information.


Thanks to the ENCODE Consortium, the ENCODE production laboratories, and the ENCODE Data Coordination Center for generating and processing the datasets used here. The ENCODE accession numbers of the constituent datasets are available from the peak details page. Henry Pratt, Jill Moore, Michael Purcaro, and Zhiping Weng, PI, at the ENCODE Data Analysis Center (ZLab at UMass Medical Center) developed this trackhub. Special thanks to Kate Rosenbloom and Jim Kent at UCSC for guidance developing this trackhub.


ENCODE Project Consortium, Jill E. Moore, Michael J. Purcaro, Henry E. Pratt, Charles B. Epstein, Noam Shoresh, Jessika Adrian, et al. 2020. "Expanded Encyclopaedias of DNA Elements in the Human and Mouse Genomes." Nature 583 (7818): 699–710.


Please contact henry.pratt@umassmed.edu with any questions or comments about this trackhub.